For RNA-Seq, it has been challenging to distinguish PCR duplicates from biological duplicates. Ghost found a tool that tackles this and...

We may occasionally find unexpected amount of poly-G reads in raw data generated from NextSeq. See FASTQC figure below as a typical...

During the standard library preparation, we usually use physical or enzymatic methods to fragment DNA. How different fragment size...

Copy number variations (CNVs) have been well studied regarding their association with tumorigenesis. Meanwhile, numerous targeted...

bam file may store more alignment information than you think. As probably the most important format in NGS analysis, almost all sorts of...

Assuming we suspect that a retrovirus or artificial vector integrated into human genome, how can we find out the exact chromosome...

Note that the statistics we are going to talk about only applies to germline mutation, not somatic mutation. Once we called a list of...

Earlier I started a discussion regarding Duplicates issue in NGS. I later had some further thoughts about this topic and would like to...

In previous POST, we discussed several possible bias sources and how to correct them. Here Ghost wanna talk about two other bias that...

Figure below very well explains two important attributes of a variant: population frequency (to avoid confusion, we use "population...